This product allows you to upgrade your classical microbiological laboratory to RT-PCR laboratory. This upgrade package includes all the tools you need: equipments, specific software parts for nucleic acid purification and detection, installation and training. For further information please contact us.
Products
FUN LC RT-PCR Workflow System Kit
This kit allows you the rapid detection of viable yeasts and moulds from beverage products like juices, teas, energy drinks or concentrates. The workflow system uses automated nucleic acid purification, automated PCR set up and automated detection and identification in results reporting. The results can be gained within 3 hours from colonies and 2,5 days from final beverage products. The throughput of the system is 60 bottles per 1,5 hour.
This kit detects all yeasts and moulds, relevant yeasts and moulds can be distinguished e.g. Candida parapsilosis, Candida pseudointermedia, Debaryomyces hansenii, Dekkera spp., Metschnikowia pulcherrima, Saccharomyces cerevisiae, Zygosaccharomyces spp., Yarrowia lipolitica, Aspergillus spp., Mucor spp., Paecylomyces spp., Penicillium spp., Rhizopus spp., Talaromyces spp., Trichoderma spp., Ulocladium spp.
Differentiation of yeasts and moulds
Detection of yeasts and moulds from filterable and non-filterable beverage products. Identification of relevant yeasts and moulds. Detection and identification of yeasts and moulds is carried out in one step.
The FUN LC RT-PCR Workflow System Kit includes:
- FUN Pre-cultivation Kit
- FUN Isolation Kit
- Yeast and Mould detection Real-Time PCR Kit.
FUN Pre-cultivation Kit is specifically developed for Yeast & Mould cultivation, enzymatic treatment and cell-disruption for non-filterable and filterable samples prior to Nucleic Acid Isolation in FUN LC RT-PCR Workflow System. Yeast & Mould are cultivated at selective conditions. After the pre-cultivation the free nucleic acids are eliminated then the intact cells are disrupted prior to nucleic acid isolation.
FUN Isolation Kit is designed for the purification of genomic DNA of fungi from bottled final beverage products, water or culture cells. The purified high molecular weight DNA is used for Yeast and Mould detection Real-Time PCR Kit.
Yeast and Mould detection Real-Time PCR Kit was specifically developed for real-time, online PCR using Hybridization Probes. Yeast and Mould DNA is amplified with specific primers designed for the conservative region of the Fungi genome. The amplicon is detected by fluorescence using a specific pair of Hybridization Probes.
Sample material: There is no need to separate any sample components (e.g. fruit fibers) before the process which separation would mean the risk to lose contaminants attached to the separated components. Our system provides you the possibility to analyze the total contents of your sample even if it is 100% fruit juice with pulp.
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- bottled final beverage products (filterable and non-filterable including 100% fruit juices with pulp)
- concentrates
- pure cultures (e.g., colonies)
- water samples
- swab samples
Key features of our system:
- Fast: Result can be gained even within 3 hours.
- Robust: 100ml can be examined even from non-filterable beverages.
- Throughput of the LC RT-PCR Workflow System: 60 bottles / 1.5 h
- Detection and identification of the contaminants in one step
- Sensitive: Even the presence of one single cell of unwanted microorganism in the sample can be detected and identified.
- Specific: While all of spoilage microorganisms are detected, harmless microorganisms out of interest do not affect the result due to specific hybridization probes.
- The same kit and protocol can be used for filterable and for non-filterable samples.
- Automated processes
- Automatic evaluation and interpretation of results
- Electronic storage of data
- Effective tool for both Quality assurance and Root Cause analysis
- Flexible system
- Reproducible results
If you would like more information about our products, please contact us.
Related documents:
ACB LC RT-PCR Workflow System Kit
MMM Beverage Partner’s ACB LC RT-PCR Workflow System allows rapid detection of viable thermo-, acidophilic bacteria belonging to the genus of Alicyclobacillus from filterable and non-filterable beverage products (juices, teas, energy drinks, concentrates). The workflow system uses automated nucleic acid purification, automated PCR set up and automated detection and identification in results reporting. The results can be gained within 3 hours from colonies and 2,5 days from final beverage products. The throughput of the system is 60 bottles per 1,5 hour. This kit detects all bacteria belonging to Alicyclobacillus genus and identifies automatically the following Alicyclobacillus species: A. acidiphilus, A. acidocaldarius, A. acidoterrestris, A. cycloheptanicus, A. contaminans, A. fastidiosus, A. herbarius, A. hesperidum, A. macrosporangiidus, A. sacchari, A. pomorum. Detection and identification of Alicyclobacillus bacteria is carried out in one step.
Identification of different Alicyclobacillus bacteria
The ACB LC RT-PCR Workflow System Kit includes:
- ACB Pre-cultivation Kit
- ACB Isolation Kit
- Alicyclobacillus detection and identification Real-Time PCR Kit
ACB Pre-cultivation Kit is specifically developed for Alicyclobacillus bacteria cultivation, enzymatic treatment and cell-disruption for non-filterable and filterable samples prior to nucleic acid Isolation. Alicyclobacillus bacteria are cultivated at selective conditions. After pre-cultivation, free nucleic acids are eliminated then the intact cells are disrupted.
ACB Isolation Kit is designed for the purification of genomic DNA of Alicyclobacilli from concentrates or bottled final beverage products, water or culture cells by using the MagNA Pure LC 2.0 Instrument. The purified high molecular weight DNA is used for Alicyclobacillus detection and identification Real-Time PCR Kit.
Alicyclobacillus detection and identification Real-Time PCR Kit is specifically developed for real-time, online PCR using Hybridization Probes. Alicyclobacillus DNA is amplified with specific primers designed for the conservative region of the Alicyclobacillus bacterial genome. The amplicon is detected by fluorescence using a specific pair of Hybridization Probes. Hybridization Probes are used for identification of the different Alicyclobacillus species.
Sample material: There is no need to separate any sample components (e.g. fruit fibers) before the process which separation would mean the risk to lose contaminants attached to the separated components. Our system provides you the possibility to analyze the total contents of your sample even if it is 100% fruit juice.
- bottled final beverage products (filterable and non-filterable including 100% fruit juices)
- concentrates
- pure cultures (e.g., colonies)
- water samples
- swab samples
Key features of our system:
- Fast: Result can be gained even within 3 hours.
- Robust: 100ml can be examined even from non-filterable beverages.
- Throughput of the LC RT-PCR Workflow System: 60 bottles / 1.5 h
- Detection and identification of the contaminants in one step
- Sensitive: Even the presence of one single cell of unwanted microorganism in the sample can be detected and identified.
- Specific: While all of spoilage microorganisms are detected, harmless microorganisms out of interest do not affect the result due to specific hybridization probes.
- The same kit and protocol can be used for filterable and for non-filterable samples.
- Automated processes
- Automatic evaluation and interpretation of results
- Electronic storage of data
- Effective tool for both Quality assurance and Root Cause analysis
- Flexible system
- Reproducible results
If you would like more information about our products, please contact us.
Related documents:
DEK LC RT-PCR Workflow System Kit
This product is developed for the identification and quantification of yeasts belonging to the Brettanomyces/Dekkera genus from filterable and non-filterable beverage products (juices, teas, soft drinks, energy drinks, concentrates and wine) The name Brettanomyces is used for the non-spore forming form of the yeast while Dekkera refers to the telemorph form. The genus is in the family of Saccharomycetaceae and includes five known species: B/D. bruxellensis, B/D. anomalus/anomala, B/D. custersianus/custersiana, B/D. nanus/nana, B/D. naardenensis. The yeast is acid-producing, when grown on glucose rich media under aerobic conditions, produces large amounts of acetic acid. Therefore, it is currently considered as a spoilage organism responsible for off-flavor production in different products such as wine, cider, beverages or dairy products. In nature, Brettanomyces can be found on the skins of fruit. It is tolerant to low pH, growth conditions show that it prefers acidic environments and room temperature but thermal inactivation is usually observed only at temperatures over 50°C. The system is based on the amplification of DNA region selectively from Brettanomyces/Dekkera and signal detection of probes constructed as hybridisation probes for the amplified product. The DEK LC RT-PCR Workflow System has been designed based on NCBI (National Center for Biotechnology Information) nucleotide database. It was blasted and tested against bacteria, moulds and yeasts other than Brettanomyces/Dekkera. In these cases no signal was detected. The DEK LC RT-PCR Workflow System Kit includes:
- DEK Pre-cultivation Kit
- DEK Isolation Kit
- Brettanomyces/Dekkera detection and identification Real-Time PCR Kit
DEK LC RT-PCR Workflow System uses automated nucleic acid purification, automated PCR set up and automated detection in results reporting. The groups bellow can be identified by the DEK LC RT-PCR Workflow System based on the melting temperature of the probes:
| Name of microorganisms | Melting temperature data, C° | Number of type strains |
| B/D.bruxellensis or B/D.anomalus/anomala | 52,8 | DSM70001 DSM70727 |
| B/D.custersianus/ custersiana | 49,6 | DSM70736 |
| B/D. nanus/ nana | 44,4 | ATCC48014 |
| B/D. naardenensis | 39,5 | DSM70743 |
Fig 1: Identification of Brettanomyces/Dekkera yeasts by DEK LC RT-PCR Workflow
- System The DEK LC RT-PCR Workflow System advantages:Selective detection and identification of viable Brettanomyces/Dekkera on species level. The detection limit is 1 cfu/200 ml product.
- Quantification of Brettanomyces/Dekkera yeasts is solved.
- System gives information on the status of cell culturability.
If you would like more information about our products, please contact us.
WAT LC RT-PCR Workflow System
Rapid Water Quality Examination Complex System
WAT LC RT-PCR Workflow System allows rapid determination of different microbiological parameters of water samples. Water quality examination system includes qualitative detection of bacteria belonging to Enterobacteriaceae family (ENB system) or to the Pseudomonas aeruginosa species (PSA system).
WAT LC RT-PCR Workflow System uses automated nucleic acid purification and PCR set up and automated detection and result reporting. Kit is optimized for 7-30 sample analyses simultaneously in a single run. Duration of the examination from sampling to results is approximately 20-24 hours including 16 hours pre-cultivation. Sample volume is 250 ml and the preparation includes a filtration step.
The WAT LC RT-PCR Workflow System Kit includes:
- WAT Pre-cultivation Kit
Sample preparation starts with a filtration step. After the filtration, the filter membrane is put onto the surface of Nutrient agar plate and put into the incubator for pre-cultivation. After the pre-cultivation the membrane is transferred to puffer in 1 ml pre-cultivation tube where it is processed henceforward: free nucleic acids are eliminated then the intact cells are disrupted prior to nucleic acid isolation.
- WAT Isolation Kit
WAT Isolation Kit is designed for the purification of genomic DNA of target bacteria from water samples using the MagNA Pure LC Instrument or the NIMBUS4 MMMBP Intrument. The purified high molecular weight DNA is used for Enterobacteriaceae detection Real-Time PCR Kit and for Pseudomonas aeruginosa detection Real-Time PCR Kit.
- Enterobacteriaceae detection Real-Time PCR Kit (ENB system kit)
The Enterobacteriaceae detection Real-Time PCR Kit is specifically developed for real-time, online PCR using Hybridization Probes. Enterobacteriaceae DNA is amplified with specific primers designed for the conservative region of the Enterobacteriaceae genome. The amplicon is detected by fluorescence using a specific pair of Hybridization Probes. Hybridization Probes are used also for distinguishing the different Enterobacteriaceae genera.
Fig 1. Melting peaks of different Enterobacteriaceae genera
- Pseudomonas aeruginosa detection Real-Time PCR Kit (PSA system kit)
The Pseudomonas aeruginosa detection Real-Time PCR Kit is based on the amplification of DNA region selectively from Pseudomonas aeruginosa and signal detection of probes constructed as Hybridisation Probes for the amplified product.
Fig 2. Detection of P. aeruginosa strains
Key features of our system:
- Fast: Result can be gained even within 3 hours.
- Throughput of the LC RT-PCR Workflow System: 60 bottles / 1.5 h
- Sensitive: Even the presence of one single cell of unwanted microorganism in the 250 ml sample can be detected.
- Specific: While all of spoilage microorganisms are detected, harmless microorganisms out of interest do not affect the result due to specific hybridization probes.
- Automated processes
- Automatic evaluation and interpretation of results
- Electronic storage of data
- Effective tool for both Quality assurance and Root Cause analysis
- Flexible system